WebMay 5, 2024 · Reagents/media. Workplace · Gentle and careful handling. · Sterilization of all items before starting. · Sterile pipettes, pipette tips and plasticware. · No touching of sterile items to non-sterilized surfaces (including gloved hands to your own skin, clothes or hair). · Pre-sterilization of all reagents and equipment. Web6.0 Reagents/Media. 6.1. 80 % Glycerol-Sterile Solution: (ex: MP Biomedical Catalog # 3055-044 or equivalent). Always use glycerol labeled “for cell culture use” or “molecular biology reagent”. Chemical-grade glycerol is not acceptable. 6.2. Sterile WFI Quality -Cell Culture Grade Water. 6.3.

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