WebNov 24, 2024 · (d) SSIIa extracted from starch granules is present as a phosphoprotein that can be dephosphorylated by APase. Conditions as in (c), except that soluble APase was used, which gives rise to the background staining and contaminants. Stromal SSIIa shown for comparison. Webstained with Phos-Tag Phosphoprotein Gel Stain (Phos-Tag) and imaged with an E-Gel Imager System using a UV light base (Invitrogen, Carlsbad, CA) (Figure 1A, top). The gel was then stained with eLuminol Protein Gel Stain and imaged with an E-Gel Imager System using a blue light base (Invitrogen) (Figure 1A, middle). Finally, the gel was stained ...

Protein Stains: Tried, True and Not Just Blue Biocompare

WebPhosphoproteins offer a unique minute-by-minute record of ongoing signal pathway events of high functional relevance to therapeutic target selection and the prediction of toxicity. Phosphorylation and dephosphorylation of structural and regulatory proteins are major intracellular control mechanisms [74]. Web6. Basic Protocol for Phos-tag TM Aqua Gel Staining: Figure 1 shows the step-by-step procedure . Step 1) After electrophoresis of the target phosphoprotein on a … sin bom sq

Phospho-Tag™ Phosphoprotein Gel Stain ABP Biosciences

WebPhos-tag ™ fluorescent stain gel. This is a reagent for phosphorylation fluorescent imaging analysis that allows for staining at physiological pH. The reagent selectively binds with … WebPhos-tag™ 540 Phosphoprotein Blot Stain (Cat. # PRD510A001KT) • Phos-tag 540 Stain Concentrate 4 vials (each 1 mL vial is enough for 4 mini-gel blots) • Phos-tag 540 Blocking Buffer (4X), 1 bottle, 100 mL • Phos-tag 540 Blot Stain Buffer (1X), 2 bottles, 200 mL each • Phos-tag 540 Wash Buffer (2X), 2 bottles, 800 mL each http://www.abpbiosciences.com/product/phos-tag-phosphoprotein-gel-stain/ rdc coins and collectibles