Hieffclone
WebCN114134170A CN202411457529.2A CN202411457529A CN114134170A CN 114134170 A CN114134170 A CN 114134170A CN 202411457529 A CN202411457529 A CN 202411457529A CN 114134170 A CN114134170 A CN 114134170A Authority CN China Prior art keywords mcs product pgl primer nos Prior art date 2024-12-02 Legal status … WebHieffClone óUniversalOneStepCloningKit 02 Component 1~3fragments 4~6fragments Negativecontrol ddH2O Upto20μL Upto20μL Upto20μL ...
Hieffclone
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Web丁香通为您提供HieffClone™P商品详情介绍：价格：￥410，货号：10912ES，品牌：Yeasen翌圣，产地：上海，详见丁香通HieffClone™P商品详情页； 丁香通 丁香园 Web1 de jun. de 2024 · this digested vector by a HieffClone Plus multi One-Step . Cloning Kit (10912ES10, Y esen, China). The T urboID . fragment was amplified by PCR from a commercial gene
Web丁香通为您提供HieffClone零背商品详情介绍：价格：￥530，货号：10907ES20，品牌：Yeasen翌圣，产地：上海，详见丁香通HieffClone零背商品详情页； Web2 de mai. de 2024 · Carbapenemase-producing Klebsiella pneumoniae (KPC) has emerged and spread throughout the world. A retrospective analysis was performed on carbapenem-resistant K. pneumoniae isolated at our teaching hospital during the period 2009–2010, when the initial outbreak occurred. To determine the mechanism(s) that underlies the …
Web20 de jan. de 2024 · All plasmids were constructed using HieffClone One Step Cloning Kit (YEASEN, 10911). Point mutations of c‐Myc (L297A, A321del, L333A, R346A, S347A, E351A, V361A, and Q365A) were generated using Hieff Mut Site‐Directed Mutagenesis Kit (YEASEN, 11003ES10). WebHSpHS10842 using the HieffClone One Step Cloning kit (Shanghai Yeasen Biotech Co., Ltd., Shanghai, China) according to the manufacturer's instructions, and transformed into BL21 (DE3) competent cells. The correct clones were screened by primer P7/P8 (Table I) and colony PCR protocol in the above steps. inoculated into 3 ml fresh LB
Web本试剂盒是利用拓扑异构酶 (Topoisomerase)高效快速连接DNA片段的原理进一步研发而成，与传统的T4连接酶相比，具有以下优势：1）快速，仅需 1-5 min即可完成连接反应。. …
Web29 de jun. de 2024 · The DNA fragment of TurboID was cloned into this digested vector by a HieffClone Plus multi One-Step Cloning Kit (10912ES10, Yesen, China). The TurboID … dave and jenny marrs christianWeb6 de mar. de 2024 · The cloning backbone PL451 and the three segments of CVA10 carrying overlapping end (15–20 bp) were amplified and seamlessly linked together in … black and decker vacuum accessoriesWeb产品描述. 本试剂盒是利用拓扑异构酶（Topoisomerase）高效快速连接DNA片段的原理进一步研发而成，与传统的T4连接酶相比，具有以下优势：1）快速，仅需1-5分钟即可完成 … black and decker under the counter toasterWebYeasenBiotechnology(Shanghai)Co.,Ltd. E-mail:[email protected] www.yeasenbiotech.com 1 HB220923 HieffCloneTMZeroTOPO-TACloningKit ProductInformation Productname Cat# Size black and decker utility storage cabinetWebRESEARCH ARTICLE T E of I C and S R I P for C A10 Min Wang1 • Jingjing Yan1 • Liuyao Zhu1 • Meng Wang1 • Lizhen Liu1 • Rui Yu1 • Ming Chen1 • Jingna Xun1 • Yuling Zhang1 • Zhigang Yi1 • Shuye Zhang1 Received: 27 September 2024/Accepted: 24 December 2024/Published online: 6 March 2024 black and decker upright vacuum cleanerWeb丁香通为您提供HieffClone™O商品详情介绍：价格：￥1 - 1680，货号：10905ES25，品牌：进口/国产，产地：德国/中国，详见丁香通HieffClone™O商品详情页； dave and jenny marrs dish setsWeb16 de dez. de 2024 · The linear DNA was cyclized into ΔKlcA HS pHS10842 using the HieffClone One Step Cloning kit (Shanghai Yeasen Biotech Co., Ltd., Shanghai, China) … dave and jenny marrs farmhouse