Dnase rq1
RQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl oligonucleotides. The enzyme is provided with 10X Reaction Buffer (400mM Tris-HCl [pH 8.0 at 25°C], 100mM MgSO 4, 10mM CaCl 2) and Stop Buffer (20mM EGTA [pH 8.0 at 25°C]) Applications WebApr 19, 2016 · DNase Treatment of RNA Samples Prior to RT-PCR: 1. Set up the DNase digestion reaction as follows: RNA in water or TE buffer 1–8µl RQ1 RNase-Free DNase 10X Reaction Buffer 1µl RQ1 RNase-Free ...
Dnase rq1
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WebFigure Legend Snippet: A. Media from transfected cells was filtered through a 0.45 micron filter and left untreated or treated for 1 hour with RQ1-RNase-free DNase (Promega). DNA from media was isolated using a Qiagen DNeasy kit. Four microliters of eluted DNA was used to detect HPV16 E7 qPCR. WebRq1 Rnase Free Dnase, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, …
WebRQ1 RNase-Free DNase, 1,000u. Products Applications Custom Resources Support About. WebRQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl oligonucleotides. The …
WebThe RQ1 DNase digestion capacity was demonstrated by the treatment of free CpG-ODN (line 6). BSA in the formulation did not affect the CpG-ODN protection (lines 9 and 10). CpG-ODN, CpG-oligodeoxynucleotides; BSA, bovine serum albumin; SDS-PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis. WebJan 20, 1998 · DNase (RQ1 RNase-free DNase, Promega) was tested by using 0.025 units/μg DNA. RNase I (Promega) was tested by digesting 10 μg of RNA with 0.65 units of RNase I for 1 h. Changes in the activity of nucleic acid-cleaving or –degrading enzymes were followed by agarose electrophoresis and densitometric analysis of the intensity of …
WebUsing TURBO™ DNase DNase I is commonly used to clear DNA contamination from RNA samples prior to RT-PCR. Conventional DNase I has a poor affinity for DNA and cleaves …
WebDNase RQ1 (Promega M6101) 10X Reaction buffer, for use with DNase RQ1 Stop solution, for use with DNase 1.5 ml microfuge tubes, autoclaved 100% Ethanol RNase free water On bench SuperScript II reverse trascriptase (Invitrogen, 18064) -20 C Oligo(dT) primer (Invitrogen, 18418) -20 C fitness first bahrenfeldWebNov 18, 2024 · The reaction products were resistant to treatment with the DNase RQ1 and were sensitive to the RNase T1, indicating that these high-molecular-weight nucleic acids were composed entirely of newly ... can i brighten my fitbit screenWebSep 19, 2007 · Two micrograms of DNAse-treated (RQ1 DNAse; Promega) total RNA was used as a template for first-strand cDNA synthesis with Superscript II (Invitrogen) and an oligo(dT) primer. The ACTIN2 (locus no. At3g18780) gene was used as a positive internal control with primers 5′-CCTTCGTCTTGATCTTGCGG-3′ and 5′ … fitness first bagWebRQ1 (RNA Qualified) RNase-Free DNase is a DNase I that degrades both double-stranded and single-stranded DNA. Supplier: Promega M6101 Catalog No. PR-M6101 $84.00 / … can i brick up my garage doorsWebRQ1 RNase-Free DNaseは、高純度に精製されたDNase I 製品で、一本鎖あるいは二本鎖DNAを分解して3'-末端に水酸基を持つオリゴヌクレオチドを生成します。 特にRNAの … fitness first balance boardWeb1. Use 1 unit of RQ1 RNase-Free DNase per microgram of RNA. For smaller amounts of RNA, use 1 unit of RQ1 RNase-Free DNase per reaction. 2. The RQ1 RNase-Free … fitness first balgowlah timetableWebKeyword:'rq1 rnase-free dnase' Showing 1-20 of 20 results for "rq1 rnase-free dnase" within Products. Products Genes Papers Technical Documents Site Content Chromatograms. Filter & Sort. All Photos (1) RNase, DNase-free. Compare Product No. Description SDS Pricing; 11119915001: from bovine pancreas: Expand. can i brighten my screen