Cytophotometric
Webparaffin. For the cytophotometric study, one slice of each testis was serially sectioned into 8-pm-thick sections that were stained following the Feulgen method; DNA hydrolysis was performed with 5 N HCI at 20°C for 1 h. In each slice the DNA content was measured in 500 spermatogonia using five randomly selected WebCytophotometric measurements of DNA in thrombopoietic megakaryocytes ...
Cytophotometric
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WebAug 23, 2024 · Growth-regulating factors (GRFs) are plant-specific transcription factors that perform important functions in plant growth and development. Herein, we identified and characterised 24 AhGRF genes in peanut (Arachis hypogaea). AhGRF family genes were divided into six classes with OLQ and WRC domains. Transcriptome expression profile … WebThe role and fate of male germ cells in planarian regeneration was studied in a population ofDugesia lugubris s.1. which provided a suitable karyological marker to distinguish …
WebFeb 1, 2003 · Cytophotometric analysis was carried out on squashed preparations after staining according to the modified Feulgen protocol [18]. The ethanol was washed from the samples with distilled water and hydrolyzed in 5 M HCl for 35 min at 22 °C. They were then stained with Schiff's reagent for 1 h in darkness at the same temperature, followed by … WebCytophotometric measurementofnuclearDNA content Analiquotofisolated epitheliawasdissociated bytreatmentwith 0.025-0-0125%pepsin (pH 1.5) for five minutes at 37°C in a water bath. The single nuclei suspensions in phosphate buffer were filtered through a nylon mesh (37 gm) and smeared on non-fluorescent glass slides with an …
WebThe role and fate of male germ cells in planarian regeneration was studied in a population ofDugesia lugubris s.1. which provided a suitable karyological marker to distinguish diploid male germ cells from triploid embryonic and somatic cells. The nuclear Feulgen-DNA content in non-replicating triploid muscle cells of the pharynx and in non-replicating male … WebFeb 4, 2016 · Medically qualified with extensive experience in human anatomy education at university level. Teaching activities are characterized by diversity of courses, multiplicity of teaching methods including problem-based and team-based learning, development of study plans, contribution to improving teaching and utilizing educational technologies in …
WebNuclear DNA content was measured by static cytophotometry in smears from breast carcinomas from 80 women, and the results were correlated with other prognostic variables and survival using multivariate statistical analysis. 49% of patients had diploid tumors and 51% hyperdiploid tumors. There was a strong correlation between DNA ploidy and …
WebJun 1, 1989 · Quantitative cytophotometric analysis may be a useful adjunct to the detection of recurrence of malignancy, prior to it becoming visible, clinically. A case is reported by which DNA cytophotometry indicated recurrence on two occasions prior to its clinical diagnosis. can a child file taxesWebCytophotometric determination of histone content in cell nuclei of proliferating and non-proliferating root meristem cells of Triticum durum.Caryologia 24, 457–61. Google Scholar Knobloch, A., Matsudaira, H. & Vendrely, R. (1957). Etude biochimique comparée des nucléohistones des différentes vertébrées. fish city pearland txWebcells and cytophotometric single cell measurements are of complementary value. Both flow cytometric and single cell cytophoto- metric DNA determinations have been shown to be of prognostic value in certain tumors (1, 4, 6, 8, 13, 16, 18). Moreover, flow cytometry allows an estima- tion of the relative S-phase rate. In many studies S- can a child file a restraining orderCytometry is the measurement of number and characteristics of cells. Variables that can be measured by cytometric methods include cell size, cell count, cell morphology (shape and structure), cell cycle phase, DNA content, and the existence or absence of specific proteins on the cell surface or in the cytoplasm. Cytometry is used to characterize and count blood cells in common blood tests such … can a child fly with chicken poxWebCytophotometry. Basic differences and similarities of flow and static cytophotometric instruments are viewed with regard to technical peculiarities and applicability in the fields … can a child divorce a parent ukWebJun 1, 1989 · Cytophotometry GERT AUER, MD, PHD, URSULA ASKENSTEN, MD, AND OLAF AHRENS, PHD Basic differences and similarities of flow and static … fish city on blue ridgeWebApr 6, 2013 · The genome size of Cyclops in cells at early stages of cleavage (up to the fifth division) and in somatic cells was estimated by static digital Feulgen cytophotometry in … can a child be a primary beneficiary